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Abstract Mud/NuMA/LIN-5 (in flies, vertebrates, and worms) is an evolutionarily conserved protein that regulates the shape and orientation of the mitotic spindle. In vertebrate cells, these functions depend on a C-terminal region called the NuMA-Tip, which (i) mediates interaction with the conserved partner protein LGN (called Pins in flies), (ii) contains a highly conserved subsequence called the NLM, and (ii) binds directly to microtubule ends. Although Mud plays a vital role in Drosophila mitosis, less is known about its structure, particularly at the C-terminus. Through sequence analysis and functional studies, we identify the Mud-Tip region and show that it is encoded by 3 consecutive exons. These exons are spliced out of several Mud isoforms, creating functionally distinct “Tipless” variants. We find that Tipless isoforms are specifically expressed in male and female gametes, where they localize to the nuclear envelope. Although Mud is known to be essential for female fertility, we demonstrate that this function does not require an intact Tip region. We also find that Mud antagonizes the localization of Lamin, a nucleoskeletal protein, in the testis, and uncover an unexpected role for Tipless Mud in promoting male fertility. Our work reveals that while the Mud-Tip is important for Mud function at mitosis, alternative splicing ensures this region is absent from Mud isoforms that perform a moonlighting role during meiosis. 

The Wide-Field Infrared Transient Explorer (WINTER) is a new time-domain instrument which will perform a seeing-limited survey of the near-infrared sky. Deployed on a dedicated 1-meter robotic telescope at Palomar Observatory, WINTER is designed to study transients of particular interest in the near-infrared including kilo-novae from gravitational-wave sources, supernovae, tidal disruption events, and transiting exoplanets around low mass stars with surveys to a depth of J=21 magnitudes. WINTER's custom camera combines six commercial large-format Indium Gallium Arsenide (InGaAs) sensors, observing in Y, J, and a short-H (Hs) band filters (0.9-1.7 microns), and employs a novel tiled optical design to cover a >1 degree squared field of view with 90% fill factor. Each wide-format (1920 x 1080 pixels) InGaAs sensor operates at T = -50°C with a thermoelectric cooler, achieving background-limited photometry without cryogenic cooling. The tiled InGaAs sensors result in a wide field-of-view instrument with significant cost savings when compared to HgCdTe sensors. We present WINTER's novel readout scheme, which includes custom electronics, firmware, and software for low-noise, real-time readout of the InGaAs sensors, including up to a 30x speed up of data reduction using GPUs. This work also outlines the cooling design for warm (T = -50°C) operation of the sensors with a two-stage thermometric cooler, copper heat pipes, and liquid cooling. We conclude with updates on the alignment, integration, and test of the WINTER instrument with a projected first light in Fall 2022. 

We present the InGaAs detector system of the Wide-Field Infrared Transient Explorer (WINTER), a new infrared instrument operating on a 1 meter robotic telescope at the Palomar Observatory. These commercially produced sensors are cooled to -50 °C by a thermo-electric cooler integrated into a room temperature package. These warm InGaAs sensors represent a dramatic reduction in cost and complexity over HgCdTe systems and achieve sky background-limited performance across our science bands for exposures greater than a few seconds. We present the design and implementation of the WINTER detector system and readout electronics.